TABLE 5.10

Calculation exercise for possible bioreactor needs

Vaccine type

Dosage (infect. units/

dose) (total virions/dose)

Doses per

year

Total viruses

needed

CSVY

(virus/cell)

Total cells

needed

Vessel

Max. cells/

vessel 1

Vessel

wv (L) 1

Vessel units

needed 2

Wv

needed (L) 2

Live-atten.

1E07

1E07

1E14

150

6.7E11

RB

8.5E07

0.26

1.6E04

4078

Live-atten.

1E07

1E07

1E14

150

6.7E11

cc

8.5E08

0.60

1.6E03

941

Live-atten.

1E07

1E07

1E14

150

6.7E11

ic

1.5E12

70

1

62

Live-atten.

1E07

1E07

1E14

150

6.7E11

STR

2.0E09

1

667

667

Live-atten.

1E07

1E07

1E14

150

6.7E11

pSTR

5.0E10

1

27

27

Live-atten.

1E07

8E10

8E17

150

5.3E15

RB

8.5E07

0.26

1.3E08

3.3E07

Live-atten.

1E07

8E10

8E17

150

5.3E15

cc

8.5E08

0.60

1.3E07

7.5E06

Live-atten.

1E07

8E10

8E17

150

5.3E15

ic

1.5E12

70

7.1E03

5.0E05

Live-atten.

1E07

8E10

8E17

150

5.3E15

STR

2.0E09

1

5.3E06

5.3E06

Live-atten.

1E07

8E10

8E17

150

5.3E15

pSTR

5.0E10

1

2.1E05

2.1E05

Live-atten.

1E07

8E10

8E17

20

4.0E16

RB

8.5E07

0.26

9.4E08

2.5E08

Live-atten.

1E07

8E10

8E17

20

4.0E16

cc

8.5E08

0.60

9.4E07

5.6E07

Live-atten.

1E07

8E10

8E17

20

4.0E16

ic

1.5E12

70

5.3E04

3.7E06

Live-atten.

1E07

8E10

8E17

20

4.0E16

STR

2.0E09

1

4.0E07

4.0E07

Live-atten.

1E07

8E10

8E17

20

4.0E16

pSTR

5.0E10

1

1.6E06

1.6E06

Inactivated

1E09

8E10

8E19

1.5E04

5.3E15

RB

8.5E07

0.26

1.3E08

3.3E07

Inactivated

1E09

8E10

8E19

1.5E04

5.3E15

cc

8.5E08

0.60

1.3E07

7.5E06

Inactivated

1E09

8E10

8E19

1.5E04

5.3E15

ic

1.5E12

70

7.1E03

5.0E05

Inactivated

1E09

8E10

8E19

1.5E04

5.3E15

STR

2.0E09

1

5.3E06

5.3E06

Inactivated

1E09

8E10

8E19

1.5E04

5.3E15

pSTR

5.0E10

1

2.1E05

2.1E05

Notes

1 For each vessel, the maximum cells per vessel unit and vessel working volume (see Table 5.6). RB: roller bottle (850 cm2); STR: stirred tank bioreactor (1 L wv); pSTR:

perfusion STR (1 L wv); cc: cell cube; ic: icellis max. For pSTR additional medium for perfusion is needed. Cultivation either of suspension or of adherent cells is

possible in the vessel.

2 To consider 50% loss in DSP all values are multiplied by two.

(These calculations assume that cell specific virus yield (CSVY) is the same in each vessel. This is often not the case.)

126

Bioprocessing of Viral Vaccines